Hotspot

trnC-GCA-petN

LSC · 9504-10313 · 810 bp

Back to Cremanthodium

Pi 0.00877

MarkerSeek score 61.30

Primer available yes

Label trnC-GCA-petN

Feature Metadata

label_name: trnC-GCA-petN
feature_type: igs
region: LSC
length_bp: 810
variable_sites: 13
pi: 0.00877
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-GCA-petN_p1	GTTGTGTATCGTTTTGGCGG	AGCCCAAGCGAGACTTACTA	50.0	50.0	58.0	58.2	915-924 bp	1.0	81.4
trnC-GCA-petN_p2	TTTCCCCAGTTCAAATCCGG	AGCCCAAGCGAGACTTACTA	50.0	50.0	58.1	58.2	858-867 bp	1.0	81.3
trnC-GCA-petN_p3	GGGGGACTGCAAATCCTTTT	AGCCCAAGCGAGACTTACTA	50.0	50.0	58.4	58.2	876-885 bp	1.0	79.0
trnC-GCA-petN_p4	TGTTGTGTATCGTTTTGGCG	AGCCCAAGCGAGACTTACTA	45.0	50.0	57.3	58.2	916-925 bp	1.0	76.4
trnC-GCA-petN_p5	GTTGTGTATCGTTTTGGCGG	GCCCAAGCGAGACTTACTAT	50.0	50.0	58.0	57.1	914-923 bp	1.0	75.5