primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
trnC-GCA-petN_p1	trnC-GCA-petN	1	GTTGTGTATCGTTTTGGCGG	AGCCCAAGCGAGACTTACTA	20	20	50.0	50.0	58.0	58.2	0.0	0.0	0.192931	9504	10313	915-924	1.000000	81.4
trnC-GCA-petN_p2	trnC-GCA-petN	2	TTTCCCCAGTTCAAATCCGG	AGCCCAAGCGAGACTTACTA	20	20	50.0	50.0	58.1	58.2	0.0	0.0	0.233476	9504	10313	858-867	1.000000	81.3
trnC-GCA-petN_p3	trnC-GCA-petN	3	GGGGGACTGCAAATCCTTTT	AGCCCAAGCGAGACTTACTA	20	20	50.0	50.0	58.4	58.2	14.7	0.0	0.506839	9504	10313	876-885	1.000000	79.0
trnC-GCA-petN_p4	trnC-GCA-petN	4	TGTTGTGTATCGTTTTGGCG	AGCCCAAGCGAGACTTACTA	20	20	45.0	50.0	57.3	58.2	0.0	0.0	0.823536	9504	10313	916-925	1.000000	76.4
trnC-GCA-petN_p5	trnC-GCA-petN	5	GTTGTGTATCGTTTTGGCGG	GCCCAAGCGAGACTTACTAT	20	20	50.0	50.0	58.0	57.1	0.0	0.0	0.934367	9504	10313	914-923	1.000000	75.5
atpH-atpF_p1	atpH-atpF	1	CGAGGCGGAGGGAAAAATAA	TGGCCCAAAGAAACGAAAGA	20	20	50.0	45.0	58.0	57.9	0.0	0.0	0.092731	26471	26866	548-552	1.000000	81.0
atpH-atpF_p2	atpH-atpF	2	CGAGGCGGAGGGAAAAATAA	ATTAAACCCGAAACTCCCGG	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.150994	26471	26866	579-583	1.000000	80.4
atpH-atpF_p3	atpH-atpF	3	CGAGGCGGAGGGAAAAATAA	GTGGCCCAAAGAAACGAAAG	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.166882	26471	26866	549-553	1.000000	80.4
atpH-atpF_p4	atpH-atpF	4	CGAGGCGGAGGGAAAAATAA	AGTGGCCCAAAGAAACGAAA	20	20	50.0	45.0	58.0	58.2	0.0	0.0	0.262386	26471	26866	550-554	1.000000	79.6
atpH-atpF_p5	atpH-atpF	5	GCCTGGTTGTAGCATTAGCA	TGGCCCAAAGAAACGAAAGA	20	20	50.0	45.0	58.2	57.9	0.0	0.0	0.309857	26471	26866	477-481	1.000000	79.5
atpF-atpA_p1	atpF-atpA	1	ATATTGGCATATTGGGGGCG	GTCGGCTTGAATGGTTACCA	20	20	50.0	50.0	58.1	58.2	0.0	6.5	0.332622	28127	28199	134-139	1.000000	80.3
atpF-atpA_p2	atpF-atpA	2	ATATTGGCATATTGGGGGCG	ATTTCGTCGGCTTGAATGGT	20	20	50.0	45.0	58.1	58.2	0.0	0.0	0.327480	28127	28199	139-144	1.000000	80.2
atpF-atpA_p3	atpF-atpA	3	ATATTGGCATATTGGGGGCG	CAATGCCGTCACCTACTTGA	20	20	50.0	50.0	58.1	57.9	0.0	0.0	0.242002	28127	28199	231-236	1.000000	79.8
atpF-atpA_p4	atpF-atpA	4	ATATTGGCATATTGGGGGCG	TCGTCGGCTTGAATGGTTAC	20	20	50.0	50.0	58.1	58.3	0.0	0.0	0.431502	28127	28199	136-141	1.000000	79.4
atpF-atpA_p5	atpF-atpA	5	ATATTGGCATATTGGGGGCG	GCTCAATACGTTCGCGGATA	20	20	50.0	50.0	58.1	58.2	0.0	6.1	0.386277	28127	28199	168-173	1.000000	79.3
ycf3-trnS-GGA_p1	ycf3-trnS-GGA	1	TTCTCCTGAAGTTGTCGGAA	ATGCTACGCTTTCAACCACT	20	20	45.0	45.0	56.4	57.8	0.0	0.0	1.769175	44032	44923	1019-1023	1.000000	66.6
ycf3-trnS-GGA_p2	ycf3-trnS-GGA	2	TTTCTCCTGAAGTTGTCGGA	ATGCTACGCTTTCAACCACT	20	20	45.0	45.0	56.4	57.8	0.0	0.0	1.769175	44032	44923	1020-1024	1.000000	66.6
ycf3-trnS-GGA_p3	ycf3-trnS-GGA	3	TTCTCCTGAAGTTGTCGGAA	GGATTCGAACCCTCGGTAAG	20	20	45.0	55.0	56.4	57.8	0.0	0.0	1.804650	44032	44923	1062-1066	1.000000	66.2
ycf3-trnS-GGA_p4	ycf3-trnS-GGA	4	TTTCTCCTGAAGTTGTCGGA	GGATTCGAACCCTCGGTAAG	20	20	45.0	55.0	56.4	57.8	0.0	0.0	1.804650	44032	44923	1063-1067	1.000000	66.2
ycf3-trnS-GGA_p5	ycf3-trnS-GGA	5	TTCTCCTGAAGTTGTCGGAA	CCAATGCTACGCTTTCAACC	20	20	45.0	50.0	56.4	57.7	0.0	0.0	1.846743	44032	44923	1022-1026	1.000000	65.9
trnT-UGU-trnL-UAA_p1	trnT-UGU-trnL-UAA	1	GCGATGCTCTAACCTCTGAG	CCGTAGCGTCTACCGATTTC	20	20	55.0	55.0	57.9	58.2	0.0	10.2	0.381189	46354	46978	686-686	1.000000	78.6
trnT-UGU-trnL-UAA_p2	trnT-UGU-trnL-UAA	2	GGAATCGAACCGATGACCAT	CCGTAGCGTCTACCGATTTC	20	20	50.0	55.0	57.8	58.2	15.5	10.2	0.488942	46354	46978	718-718	1.000000	77.6
trnT-UGU-trnL-UAA_p3	trnT-UGU-trnL-UAA	3	GCGATGCTCTAACCTCTGAG	CGTCTACCGATTTCGCCATA	20	20	55.0	50.0	57.9	57.6	0.0	0.0	0.543526	46354	46978	680-680	1.000000	77.3
trnT-UGU-trnL-UAA_p4	trnT-UGU-trnL-UAA	4	GGAATCGAACCGATGACCAT	CGTCTACCGATTTCGCCATA	20	20	50.0	50.0	57.8	57.6	15.5	0.0	0.651279	46354	46978	712-712	1.000000	76.4
trnT-UGU-trnL-UAA_p5	trnT-UGU-trnL-UAA	5	GCGATGCTCTAACCTCTGAG	CGTAGCGTCTACCGATTTCG	20	20	55.0	55.0	57.9	58.6	0.0	10.2	0.749620	46354	46978	685-685	1.000000	75.6
ndhC-trnV-UAC_p1	ndhC-trnV-UAC	1	CCCTTTGCTAATCGGAGCTA	AGAAGGTCTACGGTTCGAGT	20	20	50.0	50.0	57.1	57.8	0.0	0.0	1.107971	50346	51114	906-913	1.000000	72.7
ndhC-trnV-UAC_p2	ndhC-trnV-UAC	2	TCGGAGCTAAAAATCCCGAA	AGAAGGTCTACGGTTCGAGT	20	20	45.0	50.0	56.9	57.8	7.9	0.0	1.260777	50346	51114	895-902	1.000000	71.5
ndhC-trnV-UAC_p3	ndhC-trnV-UAC	3	CCCTTTGCTAATCGGAGCTAA	AGAAGGTCTACGGTTCGAGT	21	20	47.6	50.0	57.8	57.8	0.0	0.0	1.391288	50346	51114	906-913	1.000000	70.5
ndhC-trnV-UAC_p4	ndhC-trnV-UAC	4	CCCTTTGCTAATCGGAGCTA	GAAGGTCTACGGTTCGAGTC	20	20	50.0	55.0	57.1	57.5	0.0	0.0	1.451743	50346	51114	905-912	1.000000	70.0
ndhC-trnV-UAC_p5	ndhC-trnV-UAC	5	GGCCCTTTGCTAATCGGAG	AGAAGGTCTACGGTTCGAGT	19	20	57.9	50.0	58.3	57.8	0.0	0.0	1.489381	50346	51114	908-915	1.000000	69.7
rbcL-accD_p1	rbcL-accD	1	AGGCTACCAAATGGAGTCCT	AGCCTACATCTGTGTTCGAAC	20	21	50.0	47.6	58.0	58.0	11.3	21.4	1.066593	56302	56822	747-753	1.000000	72.4
rbcL-accD_p2	rbcL-accD	2	AGGCTACCAAATGGAGTCCT	GCCTACATCTGTGTTCGAACT	20	21	50.0	47.6	58.0	58.0	11.3	27.3	1.066593	56302	56822	746-752	1.000000	72.4
rbcL-accD_p3	rbcL-accD	3	GGCTACCAAATGGAGTCCTG	AGCCTACATCTGTGTTCGAAC	20	21	55.0	47.6	58.0	58.0	11.3	21.4	1.083303	56302	56822	746-752	1.000000	72.3
rbcL-accD_p4	rbcL-accD	4	GGCTACCAAATGGAGTCCTG	GCCTACATCTGTGTTCGAACT	20	21	55.0	47.6	58.0	58.0	11.3	27.3	1.083303	56302	56822	745-751	1.000000	72.3
rbcL-accD_p5	rbcL-accD	5	GAGTCCTGAACTAGCTGCTG	GCCTACATCTGTGTTCGAACT	20	21	55.0	47.6	57.7	58.0	13.4	27.3	1.331284	56302	56822	733-739	1.000000	70.3
petA-psbJ_p1	petA-psbJ	1	CGAGAAGGTTCAATTGTCCG	ATTCCGGATTGGGTTCATCC	20	20	50.0	50.0	56.8	57.6	5.1	4.1	1.527465	62320	63083	819-835	1.000000	68.7
petA-psbJ_p2	petA-psbJ	2	CGCATCTGTTATTTTGGCACA	ATTCCGGATTGGGTTCATCC	21	20	42.9	50.0	57.8	57.6	0.0	4.1	1.579670	62320	63083	870-886	1.000000	68.2
petA-psbJ_p3	petA-psbJ	3	CGAGAAGGTTCAATTGTCCGA	ATTCCGGATTGGGTTCATCC	21	20	47.6	50.0	58.3	57.6	5.1	4.1	1.681005	62320	63083	819-835	1.000000	67.4
petA-psbJ_p4	petA-psbJ	4	TCGAGAAGGTTCAATTGTCCG	ATTCCGGATTGGGTTCATCC	21	20	47.6	50.0	58.3	57.6	5.1	4.1	1.681005	62320	63083	820-836	1.000000	67.4
petA-psbJ_p5	petA-psbJ	5	CGAGAAGGTTCAATTGTCCG	TGTGATCGGTTTAGTAGGCG	20	20	50.0	50.0	56.8	57.4	5.1	0.0	1.734683	62320	63083	857-873	1.000000	67.0
psbE-petL_p1	psbE-petL	1	CAACCCGCAATGAATAGGGA	GTCGTATCTTGCTCAGACCA	20	20	50.0	50.0	57.9	57.1	6.4	0.0	0.992787	63875	65099	1417-1429	1.000000	72.8
psbE-petL_p2	psbE-petL	2	AGAACGTTCTCCTGTGCTTC	GTCGTATCTTGCTCAGACCA	20	20	50.0	50.0	57.8	57.1	24.9	0.0	1.097796	63875	65099	1340-1352	1.000000	72.0
psbE-petL_p3	psbE-petL	3	ACGAATAACCAACCCGCAAT	GTCGTATCTTGCTCAGACCA	20	20	45.0	50.0	58.2	57.1	0.0	0.0	1.112474	63875	65099	1426-1438	1.000000	71.8
psbE-petL_p4	psbE-petL	4	TGCTGACGAATAACCAACCC	GTCGTATCTTGCTCAGACCA	20	20	50.0	50.0	58.2	57.1	0.0	0.0	1.125378	63875	65099	1431-1443	1.000000	71.7
psbE-petL_p5	psbE-petL	5	CGAATAACCAACCCGCAATG	GTCGTATCTTGCTCAGACCA	20	20	50.0	50.0	57.8	57.1	0.0	0.0	1.128804	63875	65099	1425-1437	1.000000	71.7
clpP_p1	clpP	1	ATTGACGCGAAATACAGGCT	AAGGCTTTGTTACGTTTCCA	20	20	45.0	40.0	58.0	55.8	15.9	0.0	2.233911	69131	71149	2103-2113	1.000000	62.6
clpP_p2	clpP	2	CTCGGCAGGGTTAACTTCAA	AAGGCTTTGTTACGTTTCCA	20	20	50.0	40.0	57.8	55.8	5.4	0.0	2.388647	69131	71149	2130-2140	1.000000	61.3
clpP_p3	clpP	3	AACTCGGCAGGGTTAACTTC	AAGGCTTTGTTACGTTTCCA	20	20	50.0	40.0	57.8	55.8	0.0	0.0	2.390167	69131	71149	2132-2142	1.000000	61.3
clpP_p4	clpP	4	ATTGACGCGAAATACAGGCT	AAAGGCTTTGTTACGTTTCCA	20	21	45.0	38.1	58.0	56.6	15.9	12.5	2.455246	69131	71149	2104-2114	1.000000	60.8
clpP_p5	clpP	5	ATTGACGCGAAATACAGGCT	AGGCTTTGTTACGTTTCCAT	20	20	45.0	40.0	58.0	55.6	15.9	0.0	2.467727	69131	71149	2102-2112	1.000000	60.7
rps8-rpl14_p1	rps8-rpl14	1	ACTCGAACCACGCTTTTTCT	GAACTCGAGTTTTTGGTGCG	20	20	45.0	50.0	58.1	58.0	0.0	30.2	0.069252	79670	79872	362-366	1.000000	80.5
rps8-rpl14_p2	rps8-rpl14	2	ACTCGAACCACGCTTTTTCT	CGATTGCCCGAGAATTGAGA	20	20	45.0	50.0	58.1	58.1	0.0	0.0	0.110560	79670	79872	344-348	1.000000	80.3
rps8-rpl14_p3	rps8-rpl14	3	ACTCGAACCACGCTTTTTCT	TTGCCCGAGAATTGAGACAG	20	20	45.0	50.0	58.1	57.9	0.0	0.0	0.149676	79670	79872	341-345	1.000000	80.0
rps8-rpl14_p4	rps8-rpl14	4	ACTCGAACCACGCTTTTTCT	GCCCGAGAATTGAGACAGTT	20	20	45.0	50.0	58.1	57.9	0.0	0.0	0.151139	79670	79872	339-343	1.000000	80.0
rps8-rpl14_p5	rps8-rpl14	5	ACTCGAACCACGCTTTTTCT	ACTCGAGTTTTTGGTGCGAT	20	20	45.0	45.0	58.1	58.1	0.0	13.6	0.171582	79670	79872	360-364	1.000000	79.7
ycf1_p1	ycf1	1	CCCCGAACTTCTTCGTAGTG	GCGGTGGTCGTATTTCTTCT	20	20	55.0	50.0	58.0	58.0	2.5	0.0	0.013801	107613	108215	921-942	1.000000	82.1
ycf1_p2	ycf1	2	CCCGAACTTCTTCGTAGTGG	GCGGTGGTCGTATTTCTTCT	20	20	55.0	50.0	58.0	58.0	2.5	0.0	0.013801	107613	108215	920-941	1.000000	82.1
ycf1_p3	ycf1	3	GAGCTACTGAGGAACAACGG	GCGGTGGTCGTATTTCTTCT	20	20	55.0	50.0	58.0	58.0	0.0	0.0	0.008301	107613	108215	1016-1037	1.000000	82.0
ycf1_p4	ycf1	4	GGCAGAATAGCAGAGCAAGT	GCGGTGGTCGTATTTCTTCT	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.041501	107613	108215	1313-1334	1.000000	81.2
ycf1_p5	ycf1	5	GTCCCCCATCAGCGTATTTT	GCGGTGGTCGTATTTCTTCT	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.058753	107613	108215	1373-1394	1.000000	81.0
ndhF-rpl32_p1	ndhF-rpl32	1	GTTGCCGTCGGAAAAAGAAG	TAACGCTGCCCAAAATCCTT	20	20	50.0	45.0	58.0	58.1	17.5	0.0	0.114542	110438	111508	1045-1256	1.000000	84.6
ndhF-rpl32_p2	ndhF-rpl32	2	GTTGCCGTCGGAAAAAGAAG	TTAACGCTGCCCAAAATCCT	20	20	50.0	45.0	58.0	58.1	17.5	0.0	0.114542	110438	111508	1046-1257	1.000000	84.6
ndhF-rpl32_p3	ndhF-rpl32	3	TGCCGTCGGAAAAAGAAGAA	TAACGCTGCCCAAAATCCTT	20	20	45.0	45.0	58.0	58.1	17.5	0.0	0.125831	110438	111508	1043-1254	1.000000	84.5
ndhF-rpl32_p4	ndhF-rpl32	4	TGCCGTCGGAAAAAGAAGAA	TTAACGCTGCCCAAAATCCT	20	20	45.0	45.0	58.0	58.1	17.5	0.0	0.125831	110438	111508	1044-1255	1.000000	84.5
ndhF-rpl32_p5	ndhF-rpl32	5	TTGCCGTCGGAAAAAGAAGA	TAACGCTGCCCAAAATCCTT	20	20	45.0	45.0	58.0	58.1	17.5	0.0	0.125831	110438	111508	1044-1255	1.000000	84.5
ndhA_p1	ndhA	1	TGAACTCCGTTACCATAGGGA	TCATTATGGGAGAAGTTGATCGT	21	23	47.6	39.1	57.9	57.4	0.0	0.0	4.718843	117940	120088	2273-2282	1.000000	42.8
ndhA_p2	ndhA	2	ACTCCGTTACCATAGGGAACA	TCATTATGGGAGAAGTTGATCGT	21	23	47.6	39.1	58.2	57.4	11.4	0.0	4.790365	117940	120088	2270-2279	1.000000	42.3
ndhA_p3	ndhA	3	TGAACTCCGTTACCATAGGGA	TGGGAGAAGTTGATCGTTAA	21	20	47.6	40.0	57.9	54.0	0.0	0.0	5.096959	117940	120088	2267-2276	1.000000	40.6
ndhA_p4	ndhA	4	TGAACTCCGTTACCATAGGGA	TCATTATGGGAGAAGTTGATCGTT	21	24	47.6	37.5	57.9	58.0	0.0	0.0	5.115042	117940	120088	2273-2282	1.000000	40.6
ndhA_p5	ndhA	5	TGAACTCCGTTACCATAGGGA	CATTATGGGAGAAGTTGATCGT	21	22	47.6	40.9	57.9	56.0	0.0	0.0	5.139856	117940	120088	2272-2281	1.000000	40.6
ycf1_p1	ycf1	1	TGTATCTTAATGGACGATTCC	ACTGAACAAACTCGACAAAATGG	21	23	38.1	39.1	52.9	58.1	0.0	0.0	9.222132	126027	126146	197-200	1.000000	44.5
ycf1_p2	ycf1	2	TGTATCTTAATGGACGATTCC	CTGAACAAACTCGACAAAATGGA	21	23	38.1	39.1	52.9	57.8	0.0	0.0	9.311034	126027	126146	196-199	1.000000	44.5
ycf1_p3	ycf1	3	TGTATCTTAATGGACGATTCC	TGAACAAACTCGACAAAATGGA	21	22	38.1	36.4	52.9	56.8	0.0	0.0	9.379327	126027	126146	195-198	1.000000	44.5
ycf1_p4	ycf1	4	TGTATCTTAATGGACGATTCC	ACCGTCACCCATTTTTAGTAAGA	21	23	38.1	39.1	52.9	57.8	0.0	0.0	9.374210	126027	126146	221-224	1.000000	44.0
ycf1_p5	ycf1	5	TGTATCTTAATGGACGATTCC	CGACAAAATGGAAGAAGAAGAGG	21	23	38.1	43.5	52.9	57.7	0.0	0.0	9.431051	126027	126146	185-185	0.800000	33.3