Hotspot

ycf3

LSC · 41485-43521 · 2037 bp

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Pi 0.00246

MarkerSeek score 45.00

Primer available yes

Label ycf3

Feature Metadata

label_name: ycf3
feature_type: gene
region: LSC
length_bp: 2037
variable_sites: 5
pi: 0.00246
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 45.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf3_p1	CGCGCGAGTCTTTCTTACTA	CCCTCGGTAAACAAAAGCCT	50.0	50.0	57.8	58.1	2994-2996 bp	1.0	78.3
ycf3_p2	GAGCGGCTTCAGTTCTTACA	TGGTCGGGTTCAAGGATTTC	50.0	50.0	57.9	57.8	2745-2747 bp	1.0	78.2
ycf3_p3	GAGCGGCTTCAGTTCTTACA	CTGGTCGGGTTCAAGGATTT	50.0	50.0	57.9	57.8	2746-2748 bp	1.0	78.1
ycf3_p4	GAGCGGCTTCAGTTCTTACA	TATGTTGGGGTGAAAGCTGG	50.0	50.0	57.9	57.8	2762-2764 bp	1.0	78.0
ycf3_p5	TAATCCAGGGCAAGTGTTCG	TGGTCGGGTTCAAGGATTTC	50.0	50.0	57.9	57.8	2912-2914 bp	1.0	77.9