Hotspot

ndhH-rps15

SSC · 119393-119507 · 115 bp

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Pi 0.00926

MarkerSeek score 62.30

Primer available yes

Label ndhH-rps15

Feature Metadata

label_name: ndhH-rps15
feature_type: igs
region: SSC
length_bp: 115
variable_sites: 1
pi: 0.00926
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 62.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhH-rps15_p1	TCTTGTATCTGGCCCAGTCA	TTAGGAAAACGTCAACGGCT	50.0	45.0	58.0	57.8	234-241 bp	1.0	80.4
ndhH-rps15_p2	TCTTGTATCTGGCCCAGTCA	CGGCTGTTGGCTTATTTGTC	50.0	50.0	58.0	57.7	219-226 bp	1.0	80.4
ndhH-rps15_p3	AACACCATGCATTGATGGGT	TTAGGAAAACGTCAACGGCT	45.0	45.0	58.1	57.8	288-295 bp	1.0	79.8
ndhH-rps15_p4	AACACCATGCATTGATGGGT	CGGCTGTTGGCTTATTTGTC	45.0	50.0	58.1	57.7	273-280 bp	1.0	79.7
ndhH-rps15_p5	GTCAACAACATTTTCGCCGT	TTAGGAAAACGTCAACGGCT	45.0	45.0	57.9	57.8	330-337 bp	1.0	79.1