Hotspot

psaC-ndhE

SSC · 114049-114402 · 354 bp

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Pi 0.00898

MarkerSeek score 63.40

Primer available yes

Label psaC-ndhE

Feature Metadata

label_name: psaC-ndhE
feature_type: igs
region: SSC
length_bp: 354
variable_sites: 3
pi: 0.00898
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 63.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaC-ndhE_p1	TGCTTTACATCCGTCCCAAG	ACCGGCTATTGTTTCAGCAA	50.0	45.0	57.9	58.1	518-538 bp	1.0	81.9
psaC-ndhE_p2	ACATCCGTCCCAAGGTATCA	ACCGGCTATTGTTTCAGCAA	50.0	45.0	58.1	58.1	512-532 bp	1.0	81.8
psaC-ndhE_p3	TGCTTTACATCCGTCCCAAG	TGGACCGGCTATTGTTTCAG	50.0	50.0	57.9	57.9	521-541 bp	1.0	81.7
psaC-ndhE_p4	ACATCCGTCCCAAGGTATCA	TGGACCGGCTATTGTTTCAG	50.0	50.0	58.1	57.9	515-535 bp	1.0	81.6
psaC-ndhE_p5	CAAGCTCGGACACATTGAGT	ACCGGCTATTGTTTCAGCAA	50.0	45.0	58.2	58.1	472-492 bp	1.0	81.5