Hotspot

ndhF-rpl32

SSC · 108993-109704 · 712 bp

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Pi 0.0154

MarkerSeek score 67.60

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 712
variable_sites: 11
pi: 0.0154
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 67.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	CCACATACGGCGAAGTCTTA	TAAAGCGGTCCAATACCCCT	50.0	50.0	57.4	58.4	919-926 bp	1.0	73.1
ndhF-rpl32_p2	CCACATACGGCGAAGTCTTA	TTAAAGCGGTCCAATACCCC	50.0	50.0	57.4	57.6	920-927 bp	1.0	73.0
ndhF-rpl32_p3	CCACATACGGCGAAGTCTTA	CCAATACCCCTTCCCTTTCC	50.0	55.0	57.4	57.5	910-917 bp	1.0	72.8
ndhF-rpl32_p4	AAGAAGACCCAACCCTAGGA	TAAAGCGGTCCAATACCCCT	50.0	50.0	57.3	58.4	880-887 bp	1.0	71.8
ndhF-rpl32_p5	AGAAGACCCAACCCTAGGAA	TAAAGCGGTCCAATACCCCT	50.0	50.0	57.3	58.4	879-886 bp	1.0	71.8