Hotspot

petA-psbJ

LSC · 61160-61447 · 288 bp

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Pi 0.0279

MarkerSeek score 68.40

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 288
variable_sites: 8
pi: 0.0279
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 68.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	CCCATTACGTGTCCAAGGAC	CATATTCCGGATTGGGGTCA	55.0	50.0	58.3	57.1	435-756 bp	1.0	71.4
petA-psbJ_p2	GACCCATTACGTGTCCAAGG	CATATTCCGGATTGGGGTCA	55.0	50.0	58.3	57.1	437-758 bp	1.0	71.4
petA-psbJ_p3	ACGTGTCCAAGGACTTTTGT	CATATTCCGGATTGGGGTCA	45.0	50.0	57.6	57.1	429-750 bp	1.0	70.2
petA-psbJ_p4	CCCATTACGTGTCCAAGGAC	TGGTTCATATTCCGGATTGGG	55.0	47.6	58.3	57.8	440-761 bp	1.0	69.2
petA-psbJ_p5	GACCCATTACGTGTCCAAGG	TGGTTCATATTCCGGATTGGG	55.0	47.6	58.3	57.8	442-763 bp	1.0	69.2