Hotspot

atpB-rbcL

LSC · 52983-53702 · 720 bp

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Pi 0.00417

MarkerSeek score 61.10

Primer available yes

Label atpB-rbcL

Feature Metadata

label_name: atpB-rbcL
feature_type: igs
region: LSC
length_bp: 720
variable_sites: 3
pi: 0.00417
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpB-rbcL_p1	ATGATTTGGACGATACGCCC	CACCAGCTTTGAATCCAACAC	50.0	47.6	58.1	58.0	876 bp	1.0	71.2
atpB-rbcL_p2	ATGATTTGGACGATACGCCC	ACCAGCTTTGAATCCAACACT	50.0	42.9	58.1	58.1	875 bp	1.0	70.9
atpB-rbcL_p3	GGCGGAAAGGCTACATCTAG	CACCAGCTTTGAATCCAACAC	55.0	47.6	57.8	58.0	906 bp	1.0	70.9
atpB-rbcL_p4	GGCGGAAAGGCTACATCTAG	ACCAGCTTTGAATCCAACACT	55.0	42.9	57.8	58.1	905 bp	1.0	70.6
atpB-rbcL_p5	GGAACCAGAACCGGAAGTAG	CACCAGCTTTGAATCCAACAC	55.0	47.6	57.6	58.0	826 bp	1.0	69.1