Hotspot

ndhC-trnV-UAC

LSC · 49470-49919 · 450 bp

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Pi 0.0111
MarkerSeek score 52.80
Primer available yes
Label ndhC-trnV-UAC

Feature Metadata

label_name
ndhC-trnV-UAC
feature_type
igs
region
LSC
length_bp
450
variable_sites
5
pi
0.0111
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
52.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-UAC_p1 AGACTTGATATTATTAGAAATGCCCA AGAAGGTCTACGGTTCGAGT 30.8 50.0 56.5 57.8 539-541 bp 1.0 40.7
ndhC-trnV-UAC_p2 GACTTGATATTATTAGAAATGCCCA AGAAGGTCTACGGTTCGAGT 32.0 50.0 55.3 57.8 538-540 bp 1.0 40.7
ndhC-trnV-UAC_p3 AGACTTGATATTATTAGAAATGCCCA GAAGGTCTACGGTTCGAGTC 30.8 55.0 56.5 57.5 538-540 bp 1.0 40.7
ndhC-trnV-UAC_p4 AAGACTTGATATTATTAGAAATGCCCA AGAAGGTCTACGGTTCGAGT 29.6 50.0 57.1 57.8 540-542 bp 1.0 40.7
ndhC-trnV-UAC_p5 AGACTTGATATTATTAGAAATGCCCAA AGAAGGTCTACGGTTCGAGT 29.6 50.0 57.1 57.8 539-541 bp 1.0 40.7