Hotspot

petN-psbM

LSC · 27300-28442 · 1143 bp

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Pi 0.0018

MarkerSeek score 62.70

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 1143
variable_sites: 2
pi: 0.0018
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 62.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	TCTTGCTTGGGCTGCTTTAA	TCATTCTAGTTCCTACCGCCT	45.0	47.6	58.0	58.0	1250-1284 bp	1.0	74.0
petN-psbM_p2	CCCTGTCACTTGTAGTGTGG	TCATTCTAGTTCCTACCGCCT	55.0	47.6	57.8	58.0	1212-1246 bp	1.0	72.8
petN-psbM_p3	CCTGTCACTTGTAGTGTGGG	TCATTCTAGTTCCTACCGCCT	55.0	47.6	57.8	58.0	1211-1245 bp	1.0	72.8
petN-psbM_p4	CTGTCACTTGTAGTGTGGGG	TCATTCTAGTTCCTACCGCCT	55.0	47.6	57.8	58.0	1210-1244 bp	1.0	72.8
petN-psbM_p5	AGTAAGTCTTGCTTGGGCTG	TCATTCTAGTTCCTACCGCCT	50.0	47.6	57.8	58.0	1256-1290 bp	1.0	72.5