Hotspot

trnS-GCU-trnG-UCC

LSC · 6840-7411 · 572 bp

Back to Crawfurdia

Pi 0.0122

MarkerSeek score 65.20

Primer available yes

Label trnS-GCU-trnG-UCC

Feature Metadata

label_name: trnS-GCU-trnG-UCC
feature_type: igs
region: LSC
length_bp: 572
variable_sites: 7
pi: 0.0122
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnG-UCC_p1	CCGTCGCTTTAATCCACTCA	TCAAACCGAAAGCAAAGATCC	50.0	42.9	58.0	57.1	682-686 bp	1.0	65.5
trnS-GCU-trnG-UCC_p2	CCGTCGCTTTAATCCACTCA	CAAACCGAAAGCAAAGATCCT	50.0	42.9	58.0	57.1	681-685 bp	1.0	65.4
trnS-GCU-trnG-UCC_p3	TCGCTTTAATCCACTCAGCC	TCAAACCGAAAGCAAAGATCC	50.0	42.9	58.0	57.1	679-683 bp	1.0	65.3
trnS-GCU-trnG-UCC_p4	TCGCTTTAATCCACTCAGCC	CAAACCGAAAGCAAAGATCCT	50.0	42.9	58.0	57.1	678-682 bp	1.0	65.2
trnS-GCU-trnG-UCC_p5	CGGAAGAGGAAAGAGAGGGA	TCAAACCGAAAGCAAAGATCC	55.0	42.9	58.1	57.1	745-749 bp	1.0	64.3