Hotspot

psbK-psbI

LSC · 6106-6536 · 431 bp

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Pi 0.0117

MarkerSeek score 62.40

Primer available yes

Label psbK-psbI

Feature Metadata

label_name: psbK-psbI
feature_type: igs
region: LSC
length_bp: 431
variable_sites: 5
pi: 0.0117
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbK-psbI_p1	CTTTTGTTTGGCAAGCTGCT	TCTTCACGTCCAGGATTACG	45.0	50.0	58.1	57.1	573-574 bp	1.0	73.1
psbK-psbI_p2	GTTTGGCAAGCTGCTGTAAG	TCTTCACGTCCAGGATTACG	50.0	50.0	57.9	57.1	568-569 bp	1.0	73.1
psbK-psbI_p3	TGCCCATCATACCTCTGCTA	TCTTCACGTCCAGGATTACG	50.0	50.0	57.9	57.1	606-607 bp	1.0	72.6
psbK-psbI_p4	TGGCAAGCTGCTGTAAGTTT	TCTTCACGTCCAGGATTACG	45.0	50.0	58.3	57.1	565-566 bp	1.0	71.1
psbK-psbI_p5	TTGGCAAGCTGCTGTAAGTT	TCTTCACGTCCAGGATTACG	45.0	50.0	58.3	57.1	566-567 bp	1.0	71.1