Hotspot

ycf1

SSC · 126863-132736 · 5874 bp

Back to Cratoxylum

Pi 0.013

MarkerSeek score 47.80

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5874
variable_sites: 114
pi: 0.013
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 47.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	CCGCATTGGTCCACCTAATA	CTGGTGAATTTACTAATGATTGGGA	50.0	36.0	57.4	57.1	206 bp	1.0	44.3
ycf1_p2	CCGCATTGGTCCACCTAATA	TCTGGTGAATTTACTAATGATTGGG	50.0	36.0	57.4	57.1	207 bp	1.0	44.3
ycf1_p3	CCGCATTGGTCCACCTAATA	TGGTGAATTTACTAATGATTGGGA	50.0	33.3	57.4	56.1	205 bp	1.0	44.3
ycf1_p4	CCGCATTGGTCCACCTAATA	CTGGTGAATTTACTAATGATTGGG	50.0	37.5	57.4	55.8	206 bp	1.0	44.3
ycf1_p5	CCGCATTGGTCCACCTAATA	TTCTGGTGAATTTACTAATGATTGGG	50.0	34.6	57.4	57.7	208 bp	1.0	44.2