Hotspot

ndhF

SSC · 113209-115473 · 2265 bp

Back to Cratoxylum

Pi 0.0121

MarkerSeek score 50.20

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2265
variable_sites: 41
pi: 0.0121
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 50.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	CTTCCGAAACGAAGTGGACT	TTCAAGGCTGCCCAATATCC	50.0	50.0	57.8	57.9	2996 bp	0.333333	51.6
ndhF_p2	CTTCCGAAACGAAGTGGACT	CAAGGCTGCCCAATATCCTT	50.0	50.0	57.8	57.9	2994 bp	0.333333	51.5
ndhF_p3	CACACTGGAGGCAACCATAT	TTCAAGGCTGCCCAATATCC	50.0	50.0	57.6	57.9	2885 bp	0.333333	49.4
ndhF_p4	CACACTGGAGGCAACCATAT	CAAGGCTGCCCAATATCCTT	50.0	50.0	57.6	57.9	2883 bp	0.333333	49.3
ndhF_p5	CGACTATACACACTGGAGGC	TTCAAGGCTGCCCAATATCC	55.0	50.0	57.5	57.9	2893 bp	0.333333	48.8