Hotspot

ndhG-ndhI

SSC · 121283-121879 · 597 bp

Back to Cratoxylum

Pi 0.026

MarkerSeek score 65.30

Primer available yes

Label ndhG-ndhI

Feature Metadata

label_name: ndhG-ndhI
feature_type: igs
region: SSC
length_bp: 597
variable_sites: 22
pi: 0.026
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 65.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhG-ndhI_p1	GCAGCTACAAACTGGGAGTT	TTTGTGGCAATTGCGTTGAG	50.0	45.0	58.1	58.1	954-955 bp	1.0	83.1
ndhG-ndhI_p2	GCAGCTACAAACTGGGAGTT	ATTGCGTTGAGTATTGCCCA	50.0	45.0	58.1	58.2	945-946 bp	1.0	83.0
ndhG-ndhI_p3	TTTTACTGACGGGCCATAGC	TTTGTGGCAATTGCGTTGAG	50.0	45.0	58.0	58.1	1308-1309 bp	1.0	82.3
ndhG-ndhI_p4	TTTTACTGACGGGCCATAGC	ATTGCGTTGAGTATTGCCCA	50.0	45.0	58.0	58.2	1299-1300 bp	1.0	82.2
ndhG-ndhI_p5	TTTTACTGACGGGCCATAGC	TTTTGTGGCAATTGCGTTGA	50.0	40.0	58.0	57.7	1309-1310 bp	1.0	81.1