Hotspot

ndhE-ndhG

SSC · 120499-120751 · 253 bp

Back to Cratoxylum

Pi 0.0316

MarkerSeek score 65.90

Primer available yes

Label ndhE-ndhG

Feature Metadata

label_name: ndhE-ndhG
feature_type: igs
region: SSC
length_bp: 253
variable_sites: 12
pi: 0.0316
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhE-ndhG_p1	GGGCTCTAACCAGATTTCGA	GCTTTGATAGGTGCCATTGC	50.0	50.0	57.0	57.8	380-381 bp	1.0	73.0
ndhE-ndhG_p2	AGGGCTCTAACCAGATTTCG	GCTTTGATAGGTGCCATTGC	50.0	50.0	57.0	57.8	381-382 bp	1.0	72.9
ndhE-ndhG_p3	AACCAGATTTCGACTCGTGA	GCTTTGATAGGTGCCATTGC	45.0	50.0	56.9	57.8	373-374 bp	1.0	72.0
ndhE-ndhG_p4	CCAGATTTCGACTCGTGATCA	GCTTTGATAGGTGCCATTGC	47.6	50.0	57.8	57.8	371-372 bp	1.0	71.7
ndhE-ndhG_p5	ACCAGATTTCGACTCGTGATC	GCTTTGATAGGTGCCATTGC	47.6	50.0	57.8	57.8	372-373 bp	1.0	71.6