Hotspot

ycf1-ndhF

SSC · 112936-113208 · 273 bp

Back to Cratoxylum

Pi 0.0596

MarkerSeek score 71.40

Primer available yes

Label ycf1-ndhF

Feature Metadata

label_name: ycf1-ndhF
feature_type: igs
region: SSC
length_bp: 273
variable_sites: 21
pi: 0.0596
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.86
markerseek_score: 71.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1-ndhF_p1	CTTCCGAAACGAAGTGGACT	GGAGGCCGCATTTCTTCTTA	50.0	50.0	57.8	58.0	518-542 bp	1.0	86.4
ycf1-ndhF_p2	TTCGGAGAATTGGAAGACGC	GGAGGCCGCATTTCTTCTTA	50.0	50.0	58.3	58.0	583-607 bp	1.0	85.3
ycf1-ndhF_p3	GGGTTCGGATTCGGAGAATT	GGAGGCCGCATTTCTTCTTA	50.0	50.0	57.7	58.0	592-616 bp	1.0	84.8
ycf1-ndhF_p4	AGAGTCCGGCTATTCGCTTA	GGAGGCCGCATTTCTTCTTA	50.0	50.0	58.3	58.0	627-651 bp	1.0	84.7
ycf1-ndhF_p5	CTTCCGAAACGAAGTGGACT	CCCTATCCCTACGAATCCGA	50.0	55.0	57.8	57.8	1064-1088 bp	1.0	82.9