Hotspot

rrn4.5-rrn5

IRb · 109995-110230 · 236 bp

Back to Cratoxylum

Pi 0.0452

MarkerSeek score 64.70

Primer available yes

Label rrn4.5-rrn5

Feature Metadata

label_name: rrn4.5-rrn5
feature_type: igs
region: IRb
length_bp: 236
variable_sites: 16
pi: 0.0452
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rrn4.5-rrn5_p1	GTCAAGTGGAAGTGCAGTGA	ACGCCTAGGACACCAGAATA	50.0	50.0	58.0	57.8	318 bp	1.0	82.4
rrn4.5-rrn5_p2	GTCAAGTGGAAGTGCAGTGA	TACGCCTAGGACACCAGAAT	50.0	50.0	58.0	57.8	319 bp	1.0	82.4
rrn4.5-rrn5_p3	CAGCTGAGGCATCCTAACAG	GGCCTCCCCTACAGTATCTT	55.0	55.0	58.0	57.9	365 bp	1.0	82.3
rrn4.5-rrn5_p4	GCTGAGGCATCCTAACAGAC	GGCCTCCCCTACAGTATCTT	55.0	55.0	58.1	57.9	363 bp	1.0	82.3
rrn4.5-rrn5_p5	GTCAAGTGGAAGTGCAGTGA	GGCCTCCCCTACAGTATCTT	50.0	55.0	58.0	57.9	391 bp	1.0	82.1