Hotspot

rps11-rpl36

LSC · 81331-81449 · 119 bp

Back to Cratoxylum

Pi 0.023

MarkerSeek score 66.50

Primer available yes

Label rps11-rpl36

Feature Metadata

label_name: rps11-rpl36
feature_type: igs
region: LSC
length_bp: 119
variable_sites: 4
pi: 0.023
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 66.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps11-rpl36_p1	TACGTGCATTCTTACGCGAA	CCAACCCAAAGCACAAACAG	45.0	50.0	58.0	58.1	218-221 bp	1.0	81.9
rps11-rpl36_p2	TTACGTGCATTCTTACGCGA	CCAACCCAAAGCACAAACAG	45.0	50.0	58.0	58.1	219-222 bp	1.0	81.9
rps11-rpl36_p3	TACGTGCATTCTTACGCGAA	ATACGTAGACGGGGTCGAAT	45.0	50.0	58.0	58.0	252-255 bp	1.0	81.8
rps11-rpl36_p4	TTACGTGCATTCTTACGCGA	ATACGTAGACGGGGTCGAAT	45.0	50.0	58.0	58.0	253-256 bp	1.0	81.8
rps11-rpl36_p5	TACGTGCATTCTTACGCGAA	CTCCAACCCAAAGCACAAAC	45.0	50.0	58.0	57.8	220-223 bp	1.0	80.6