Hotspot

trnP-UGG-psaJ

LSC · 67972-68479 · 508 bp

Back to Cratoxylum

Pi 0.0187

MarkerSeek score 49.60

Primer available yes

Label trnP-UGG-psaJ

Feature Metadata

label_name: trnP-UGG-psaJ
feature_type: igs
region: LSC
length_bp: 508
variable_sites: 14
pi: 0.0187
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 49.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnP-UGG-psaJ_p1	ACCCGTGACATTTTGTACCC	GGCGCAACGGATAGATATGT	50.0	50.0	58.1	57.9	590-598 bp	1.0	81.5
trnP-UGG-psaJ_p2	CCCGTGACATTTTGTACCCA	GGCGCAACGGATAGATATGT	50.0	50.0	58.1	57.9	589-597 bp	1.0	81.5
trnP-UGG-psaJ_p3	ACAGGATTTGAACCCGTGAC	GGCGCAACGGATAGATATGT	50.0	50.0	58.1	57.9	601-609 bp	1.0	81.4
trnP-UGG-psaJ_p4	CAGGATTTGAACCCGTGACA	GGCGCAACGGATAGATATGT	50.0	50.0	58.1	57.9	600-608 bp	1.0	81.4
trnP-UGG-psaJ_p5	TGACAGGATTTGAACCCGTG	GGCGCAACGGATAGATATGT	50.0	50.0	58.1	57.9	603-611 bp	1.0	81.4