Hotspot

petA-psbJ

LSC · 64552-65601 · 1050 bp

Back to Cratoxylum

Pi 0.0239

MarkerSeek score 64.20

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 1050
variable_sites: 37
pi: 0.0239
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 64.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	TGAGAAGGTTCAATTGTCCGA	ATTCCGGATTGGGTTCATCC	42.9	50.0	57.2	57.6	1097-1111 bp	1.0	66.9
petA-psbJ_p2	TGAGAAGGTTCAATTGTCCGAA	ATTCCGGATTGGGTTCATCC	40.9	50.0	57.9	57.6	1097-1111 bp	1.0	64.3
petA-psbJ_p3	TTGAGAAGGTTCAATTGTCCGA	ATTCCGGATTGGGTTCATCC	40.9	50.0	57.9	57.6	1098-1112 bp	1.0	64.3
petA-psbJ_p4	TGAGAAGGTTCAATTGTCCG	ATTCCGGATTGGGTTCATCC	45.0	50.0	55.7	57.6	1097-1111 bp	1.0	62.5
petA-psbJ_p5	TTGAGAAGGTTCAATTGTCCG	ATTCCGGATTGGGTTCATCC	42.9	50.0	56.5	57.6	1098-1112 bp	1.0	60.7