Hotspot

atpH-atpF

LSC · 45232-45764 · 533 bp

Back to Cratoxylum

Pi 0.0232

MarkerSeek score 65.60

Primer available yes

Label atpH-atpF

Feature Metadata

label_name: atpH-atpF
feature_type: igs
region: LSC
length_bp: 533
variable_sites: 18
pi: 0.0232
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpF_p1	ACTGGTTGTAGCATTAGCCC	CCAGTGACCTAAGGGAACGA	50.0	55.0	57.6	58.7	619-621 bp	1.0	73.6
atpH-atpF_p2	CTGGTTGTAGCATTAGCCCT	CCAGTGACCTAAGGGAACGA	50.0	55.0	57.3	58.7	618-620 bp	1.0	71.2
atpH-atpF_p3	ACTGGTTGTAGCATTAGCCC	CAGTGACCTAAGGGAACGAA	50.0	50.0	57.6	56.9	618-620 bp	1.0	70.5
atpH-atpF_p4	GGAGGGAAAAATTCGGGGTA	CCAGTGACCTAAGGGAACGA	50.0	55.0	57.2	58.7	685-687 bp	1.0	70.2
atpH-atpF_p5	GGAGGCGGAGGGAAAAATTC	CCAGTGACCTAAGGGAACGA	55.0	55.0	58.9	58.7	691-693 bp	1.0	69.5