Hotspot

trnC-GCA-rpoB

LSC · 30613-31659 · 1047 bp

Back to Cratoxylum

Pi 0.015

MarkerSeek score 68.00

Primer available yes

Label trnC-GCA-rpoB

Feature Metadata

label_name: trnC-GCA-rpoB
feature_type: igs
region: LSC
length_bp: 1047
variable_sites: 23
pi: 0.015
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 68.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-GCA-rpoB_p1	GCGTCTTTTGTTGATCAGGC	AAGCCCTGATCAATGAACCG	50.0	50.0	58.0	58.2	1226-1464 bp	1.0	79.4
trnC-GCA-rpoB_p2	GCGTCTTTTGTTGATCAGGC	CCCTGATCAATGAACCGACA	50.0	50.0	58.0	57.6	1223-1461 bp	1.0	78.1
trnC-GCA-rpoB_p3	CGTCTTTTGTTGATCAGGCG	AAGCCCTGATCAATGAACCG	50.0	50.0	57.8	58.2	1225-1463 bp	1.0	77.7
trnC-GCA-rpoB_p4	AAAAGGATTTGCAGTCCCCC	AAGCCCTGATCAATGAACCG	50.0	50.0	58.4	58.2	1183-1421 bp	1.0	76.8
trnC-GCA-rpoB_p5	CGTCTTTTGTTGATCAGGCG	CCCTGATCAATGAACCGACA	50.0	50.0	57.8	57.6	1222-1460 bp	1.0	76.5