Hotspot

trnY-GUA-trnD-GUC

LSC · 27341-27819 · 479 bp

Back to Cratoxylum

Pi 0.0112
MarkerSeek score 58.70
Primer available yes
Label trnY-GUA-trnD-GUC

Feature Metadata

label_name
trnY-GUA-trnD-GUC
feature_type
igs
region
LSC
length_bp
479
variable_sites
8
pi
0.0112
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
58.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnY-GUA-trnD-GUC_p1 ATGGATTCTTCCTGGGTCGA CGGTGCTCTGACCAATTGAA 50.0 50.0 58.1 58.5 606-610 bp 1.0 76.4
trnY-GUA-trnD-GUC_p2 TGGATTCTTCCTGGGTCGAT CGGTGCTCTGACCAATTGAA 50.0 50.0 58.1 58.5 605-609 bp 1.0 76.4
trnY-GUA-trnD-GUC_p3 TGGCAATATGTCTACGCTGG CGGTGCTCTGACCAATTGAA 50.0 50.0 57.8 58.5 549-553 bp 1.0 75.6
trnY-GUA-trnD-GUC_p4 GGCAATATGTCTACGCTGGT CGGTGCTCTGACCAATTGAA 50.0 50.0 57.8 58.5 548-552 bp 1.0 75.6
trnY-GUA-trnD-GUC_p5 GGATTCTTCCTGGGTCGATG CGGTGCTCTGACCAATTGAA 55.0 50.0 57.8 58.5 604-608 bp 1.0 75.4