Hotspot

trnG-GCC-psbZ

LSC · 20929-21626 · 698 bp

Back to Cratoxylum

Pi 0.0193
MarkerSeek score 63.00
Primer available yes
Label trnG-GCC-psbZ

Feature Metadata

label_name
trnG-GCC-psbZ
feature_type
igs
region
LSC
length_bp
698
variable_sites
19
pi
0.0193
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.94
markerseek_score
63.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnG-GCC-psbZ_p1 CGTCTTCTCCTTGGCAAAGA AGTGGGTATCCTTAATTCTCTCA 50.0 39.1 57.8 56.2 734-776 bp 1.0 46.0
trnG-GCC-psbZ_p2 CGTCTTCTCCTTGGCAAAGA GTCTTTCTAGTGGGTATCCT 50.0 45.0 57.8 53.1 742-784 bp 1.0 46.0
trnG-GCC-psbZ_p3 CGTCTTCTCCTTGGCAAAGA GTCTTTCTAGTGGGTATCCTT 50.0 42.9 57.8 54.1 742-784 bp 1.0 46.0
trnG-GCC-psbZ_p4 CGTCTTCTCCTTGGCAAAGA GGTATCCTTAATTCTCTCATCTCGT 50.0 40.0 57.8 57.8 730-772 bp 1.0 46.0
trnG-GCC-psbZ_p5 CGTCTTCTCCTTGGCAAAGA GTGGGTATCCTTAATTCTCTCA 50.0 40.9 57.8 54.7 733-775 bp 1.0 46.0