Hotspot

trnfM-CAU-trnG-GCC

LSC · 20809-20857 · 49 bp

Back to Cratoxylum

Pi 0.0408

MarkerSeek score 74.70

Primer available yes

Label trnfM-CAU-trnG-GCC

Feature Metadata

label_name: trnfM-CAU-trnG-GCC
feature_type: igs
region: LSC
length_bp: 49
variable_sites: 3
pi: 0.0408
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 74.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnfM-CAU-trnG-GCC_p1	CTTGAGGTCACGGGTTCAAA	TCTTTGCCAAGGAGAAGACG	50.0	50.0	58.0	57.8	127 bp	1.0	80.2
trnfM-CAU-trnG-GCC_p2	TGAGGTCACGGGTTCAAATC	TCTTTGCCAAGGAGAAGACG	50.0	50.0	57.8	57.8	125 bp	1.0	79.1
trnfM-CAU-trnG-GCC_p3	GTAGCTCGCAAGGCTCATAA	TCTTTGCCAAGGAGAAGACG	50.0	50.0	57.8	57.8	148 bp	1.0	78.5
trnfM-CAU-trnG-GCC_p4	TAGCTCGCAAGGCTCATAAC	TCTTTGCCAAGGAGAAGACG	50.0	50.0	57.8	57.8	147 bp	1.0	78.5
trnfM-CAU-trnG-GCC_p5	GAGGTCACGGGTTCAAATCC	TCTTTGCCAAGGAGAAGACG	55.0	50.0	58.5	57.8	124 bp	1.0	76.2