Hotspot

ndhJ-trnF-GAA

LSC · 9999-10479 · 481 bp

Back to Cratoxylum

Pi 0.0169

MarkerSeek score 68.30

Primer available yes

Label ndhJ-trnF-GAA

Feature Metadata

label_name: ndhJ-trnF-GAA
feature_type: igs
region: LSC
length_bp: 481
variable_sites: 12
pi: 0.0169
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 68.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhJ-trnF-GAA_p1	CCGGAAAGTTGGATAGGGTG	GGGATAGCTCAGCTGGTAGA	55.0	55.0	58.0	58.0	622-629 bp	1.0	82.1
ndhJ-trnF-GAA_p2	CGGAAAGTTGGATAGGGTGG	GGGATAGCTCAGCTGGTAGA	55.0	55.0	58.0	58.0	621-628 bp	1.0	82.1
ndhJ-trnF-GAA_p3	CCGGAAAGTTGGATAGGGTG	CCTCGTGTCACCAGTTCAAA	55.0	50.0	58.0	58.1	586-593 bp	1.0	81.9
ndhJ-trnF-GAA_p4	CGGAAAGTTGGATAGGGTGG	CCTCGTGTCACCAGTTCAAA	55.0	50.0	58.0	58.1	585-592 bp	1.0	81.9
ndhJ-trnF-GAA_p5	CGGAAAGTTGGATAGGGTGG	CTGGTAGAGCAGAGGACTGA	55.0	55.0	58.0	57.9	609-616 bp	1.0	81.1