Hotspot

trnH-GUG-psbA

LSC · 69-586 · 518 bp

Back to Cratoxylum

Pi 0.0275
MarkerSeek score 70.10
Primer available yes
Label trnH-GUG-psbA

Feature Metadata

label_name
trnH-GUG-psbA
feature_type
igs
region
LSC
length_bp
518
variable_sites
15
pi
0.0275
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.73
markerseek_score
70.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnH-GUG-psbA_p1 GATCCACTTGGCTACATCCG TTCCCTCTAGACCTAGCTGC 55.0 55.0 58.1 57.9 448-587 bp 1.0 84.8
trnH-GUG-psbA_p2 GCCTTGATCCACTTGGCTAC TTCCCTCTAGACCTAGCTGC 55.0 55.0 58.6 57.9 453-592 bp 1.0 80.9
trnH-GUG-psbA_p3 GATCCACTTGGCTACATCCG CGTGCTAACCTTGGTATGGA 55.0 50.0 58.1 57.3 496-635 bp 1.0 79.7
trnH-GUG-psbA_p4 GATCCACTTGGCTACATCCG TCGTGCTAACCTTGGTATGG 55.0 50.0 58.1 57.3 497-636 bp 1.0 79.7
trnH-GUG-psbA_p5 CCTTGATCCACTTGGCTACA TTCCCTCTAGACCTAGCTGC 50.0 55.0 57.2 57.9 452-591 bp 1.0 79.5