Hotspot

rps8

LSC · 81258-81662 · 405 bp

Back to Crateva

Pi 0.0115

MarkerSeek score 48.60

Primer available yes

Label rps8

Feature Metadata

label_name: rps8
feature_type: gene
region: LSC
length_bp: 405
variable_sites: 7
pi: 0.0115
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 48.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps8_p1	TAAATCCACAAGTACCCGCG	GGGCTCGAGAATTGATGTGT	50.0	50.0	58.0	58.0	1675-1732 bp	1.0	84.0
rps8_p2	TAAATCCACAAGTACCCGCG	CCCACGGGAATTGAGACAAT	50.0	50.0	58.0	57.9	1413-1470 bp	1.0	83.9
rps8_p3	TAAATCCACAAGTACCCGCG	AGGAGCTAGTAATCGCCGAT	50.0	50.0	58.0	58.1	1644-1701 bp	1.0	83.6
rps8_p4	GGAGGACCAAGAAATCACCC	GGGCTCGAGAATTGATGTGT	55.0	50.0	57.9	58.0	1656-1713 bp	1.0	83.1
rps8_p5	GGAGGACCAAGAAATCACCC	AGGAGCTAGTAATCGCCGAT	55.0	50.0	57.9	58.1	1625-1682 bp	1.0	82.7