Hotspot

ndhF

SSC · 111888-114137 · 2250 bp

Back to Crateva

Pi 0.0111

MarkerSeek score 48.90

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2250
variable_sites: 25
pi: 0.0111
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 48.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	AGAAACGGAAGAAAGGGGTG	TTATTGCGCCCTTTGAAAAA	50.0	35.0	57.7	54.7	2563-2566 bp	1.0	55.8
ndhF_p2	AGAAACGGAAGAAAGGGGTG	TTTATTGCGCCCTTTGAAAA	50.0	35.0	57.7	54.7	2564-2567 bp	1.0	55.8
ndhF_p3	AGAAACGGAAGAAAGGGGTG	TTTTATTGCGCCCTTTGAAA	50.0	35.0	57.7	54.7	2565-2568 bp	1.0	55.8
ndhF_p4	AGAAACGGAAGAAAGGGGTG	TTTTTATTGCGCCCTTTGAA	50.0	35.0	57.7	54.7	2566-2569 bp	1.0	55.8
ndhF_p5	CAGAAACGGAAGAAAGGGGT	TTATTGCGCCCTTTGAAAAA	50.0	35.0	57.7	54.7	2564-2567 bp	1.0	55.8