Hotspot

trnL-UUG-ycf2

IRa · 146386-147351 · 966 bp

Back to Crateva

Pi 0.00989

MarkerSeek score 63.60

Primer available yes

Label trnL-UUG-ycf2

Feature Metadata

label_name: trnL-UUG-ycf2
feature_type: igs
region: IRa
length_bp: 966
variable_sites: 9
pi: 0.00989
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 63.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL-UUG-ycf2_p1	AAATGGTAGACACGCGAGAC	ACGCTATTGGATCGAATGACA	50.0	42.9	58.0	57.5	1060-1116 bp	1.0	73.0
trnL-UUG-ycf2_p2	TGAAATGGTAGACACGCGAG	ACGCTATTGGATCGAATGACA	50.0	42.9	58.0	57.5	1062-1118 bp	1.0	73.0
trnL-UUG-ycf2_p3	AAATGGTAGACACGCGAGAC	AGATGGCTTTTCCCAGATGA	50.0	45.0	58.0	56.5	1021-1077 bp	1.0	72.6
trnL-UUG-ycf2_p4	TGAAATGGTAGACACGCGAG	AGATGGCTTTTCCCAGATGA	50.0	45.0	58.0	56.5	1023-1079 bp	1.0	72.5
trnL-UUG-ycf2_p5	GCCTTGGTGGTGAAATGGTA	ACGCTATTGGATCGAATGACA	50.0	42.9	58.1	57.5	1072-1128 bp	1.0	72.4