Hotspot

ccsA-ndhD

SSC · 117104-117423 · 320 bp

Back to Crateva

Pi 0.0446

MarkerSeek score 67.30

Primer available yes

Label ccsA-ndhD

Feature Metadata

label_name: ccsA-ndhD
feature_type: igs
region: SSC
length_bp: 320
variable_sites: 14
pi: 0.0446
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 67.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA-ndhD_p1	AGGTTTACATAGTTATGGTTCA	AGCAAGTGACAAGGTCGAAT	31.8	45.0	52.5	57.4	400-406 bp	1.0	46.8
ccsA-ndhD_p2	GTCAATCTCTTAGGAATAGGTT	AGCAAGTGACAAGGTCGAAT	36.4	45.0	52.7	57.4	417-423 bp	1.0	46.7
ccsA-ndhD_p3	GTCAATCTCTTAGGAATAGGTTT	AGCAAGTGACAAGGTCGAAT	34.8	45.0	53.6	57.4	417-423 bp	1.0	46.7
ccsA-ndhD_p4	GTCAATCTCTTAGGAATAGGTTTACA	AGCAAGTGACAAGGTCGAAT	34.6	45.0	56.5	57.4	417-423 bp	1.0	46.7
ccsA-ndhD_p5	GTCAATCTCTTAGGAATAGGTT	GCAAGTGACAAGGTCGAATC	36.4	50.0	52.7	57.1	416-422 bp	1.0	46.7