Hotspot

rpl32-trnL-CUA

SSC · 115360-115929 · 570 bp

Back to Crateva

Pi 0.0282
MarkerSeek score 69.20
Primer available yes
Label rpl32-trnL-CUA

Feature Metadata

label_name
rpl32-trnL-CUA
feature_type
igs
region
SSC
length_bp
570
variable_sites
16
pi
0.0282
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.99
markerseek_score
69.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-CUA_p1 GGGATATTGGGCAGCATTGA TCCTAAGAGCAGCGTGTCTA 50.0 50.0 58.0 57.9 707-1342 bp 1.0 84.4
rpl32-trnL-CUA_p2 AGGGATATTGGGCAGCATTG TCCTAAGAGCAGCGTGTCTA 50.0 50.0 58.0 57.9 708-1343 bp 1.0 84.4
rpl32-trnL-CUA_p3 GGGATATTGGGCAGCATTGA AGCAGCGTGTCTACCAATTT 50.0 45.0 58.0 57.8 700-1335 bp 1.0 83.9
rpl32-trnL-CUA_p4 AGGGATATTGGGCAGCATTG AGCAGCGTGTCTACCAATTT 50.0 45.0 58.0 57.8 701-1336 bp 1.0 83.9
rpl32-trnL-CUA_p5 GGGATATTGGGCAGCATTGA CTAAGAGCAGCGTGTCTACC 50.0 55.0 58.0 57.8 705-1340 bp 1.0 83.8