Hotspot

ycf2-trnL-UUG

IRb · 94681-95646 · 966 bp

Back to Crateva

Pi 0.00989

MarkerSeek score 63.60

Primer available yes

Label ycf2-trnL-UUG

Feature Metadata

label_name: ycf2-trnL-UUG
feature_type: igs
region: IRb
length_bp: 966
variable_sites: 9
pi: 0.00989
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 63.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf2-trnL-UUG_p1	ACGCTATTGGATCGAATGACA	AAATGGTAGACACGCGAGAC	42.9	50.0	57.5	58.0	1060-1116 bp	1.0	73.0
ycf2-trnL-UUG_p2	ACGCTATTGGATCGAATGACA	TGAAATGGTAGACACGCGAG	42.9	50.0	57.5	58.0	1062-1118 bp	1.0	73.0
ycf2-trnL-UUG_p3	AGATGGCTTTTCCCAGATGA	AAATGGTAGACACGCGAGAC	45.0	50.0	56.5	58.0	1021-1077 bp	1.0	72.6
ycf2-trnL-UUG_p4	AGATGGCTTTTCCCAGATGA	TGAAATGGTAGACACGCGAG	45.0	50.0	56.5	58.0	1023-1079 bp	1.0	72.5
ycf2-trnL-UUG_p5	ACGCTATTGGATCGAATGACA	GCCTTGGTGGTGAAATGGTA	42.9	50.0	57.5	58.1	1072-1128 bp	1.0	72.4