Hotspot

rpl14-rpl16

LSC · 82306-82436 · 131 bp

Back to Crateva

Pi 0.0308

MarkerSeek score 67.50

Primer available yes

Label rpl14-rpl16

Feature Metadata

label_name: rpl14-rpl16
feature_type: igs
region: LSC
length_bp: 131
variable_sites: 6
pi: 0.0308
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 67.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl14-rpl16_p1	ACACATCAATTCTCGAGCCC	GGTGGGGTAGCCGAAAATAT	50.0	50.0	58.0	57.3	283-287 bp	1.0	76.5
rpl14-rpl16_p2	ATCGGCGATTACTAGCTCCT	GGTGGGGTAGCCGAAAATAT	50.0	50.0	58.1	57.3	314-318 bp	1.0	75.9
rpl14-rpl16_p3	ACACATCAATTCTCGAGCCC	TATGAAATGGGTGGGGTAGC	50.0	50.0	58.0	57.0	292-296 bp	1.0	73.3
rpl14-rpl16_p4	ATCGGCGATTACTAGCTCCT	TATGAAATGGGTGGGGTAGC	50.0	50.0	58.1	57.0	323-327 bp	1.0	72.7
rpl14-rpl16_p5	TCGGCGATTACTAGCTCCTA	GGTGGGGTAGCCGAAAATAT	50.0	50.0	57.4	57.3	313-317 bp	1.0	72.1