Hotspot

petA-psbJ

LSC · 63675-64776 · 1102 bp

Back to Crateva

Pi 0.0172

MarkerSeek score 72.10

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 1102
variable_sites: 28
pi: 0.0172
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 72.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	ATCCATTACGTGTCCAAGGC	AGGATTAGGTTCGTCCCTGT	50.0	50.0	58.0	57.8	1243-1255 bp	1.0	81.5
petA-psbJ_p2	CATTACGTGTCCAAGGCCTT	AGGATTAGGTTCGTCCCTGT	50.0	50.0	58.2	57.8	1240-1252 bp	1.0	80.4
petA-psbJ_p3	ATCCATTACGTGTCCAAGGC	TCAGGATTAGGTTCGTCCCT	50.0	50.0	58.0	57.5	1245-1257 bp	1.0	79.1
petA-psbJ_p4	TTACGTGTCCAAGGCCTTTT	AGGATTAGGTTCGTCCCTGT	45.0	50.0	57.6	57.8	1238-1250 bp	1.0	79.0
petA-psbJ_p5	ATCCATTACGTGTCCAAGGC	CAGGATTAGGTTCGTCCCTG	50.0	55.0	58.0	57.4	1244-1256 bp	1.0	78.6