Hotspot

trnF-UUC-ndhJ

LSC · 48814-49303 · 490 bp

Back to Crateva

Pi 0.0185
MarkerSeek score 62.70
Primer available yes
Label trnF-UUC-ndhJ

Feature Metadata

label_name
trnF-UUC-ndhJ
feature_type
igs
region
LSC
length_bp
490
variable_sites
13
pi
0.0185
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.99
markerseek_score
62.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnF-UUC-ndhJ_p1 CCTCGTGTCACCAGTTCAAA CCCGAAAGTTGGATAGGGTG 50.0 55.0 58.1 58.0 601-606 bp 1.0 83.0
trnF-UUC-ndhJ_p2 CCTCGTGTCACCAGTTCAAA CCGAAAGTTGGATAGGGTGG 50.0 55.0 58.1 58.0 600-605 bp 1.0 83.0
trnF-UUC-ndhJ_p3 CCTCGTGTCACCAGTTCAAA ATCCACGGCTAAAACGGATC 50.0 50.0 58.1 58.1 627-632 bp 1.0 82.7
trnF-UUC-ndhJ_p4 ATGGTCGGGATAGCTCAGTT CCCGAAAGTTGGATAGGGTG 50.0 55.0 58.2 58.0 643-648 bp 1.0 81.5
trnF-UUC-ndhJ_p5 ATGGTCGGGATAGCTCAGTT CCGAAAGTTGGATAGGGTGG 50.0 55.0 58.2 58.0 642-647 bp 1.0 81.5