Hotspot

trnP-UGG-psaJ

Genome · 70041-70480 · 440 bp

Back to Crataegus

Pi 0.00539

MarkerSeek score 50.80

Primer available yes

Label trnP-UGG-psaJ

Feature Metadata

label_name: trnP-UGG-psaJ
feature_type: igs
region: Genome
length_bp: 440
variable_sites: 19
pi: 0.00539
unique_haplotype_count: 15
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 50.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnP-UGG-psaJ_p1	TACCAAACTGCGCTACATCC	AACCTGCTAAAGACCCGAAC	50.0	50.0	58.0	57.8	502-568 bp	1.0	85.3
trnP-UGG-psaJ_p2	TACCAAACTGCGCTACATCC	GGAATATCAACGCATCCGGA	50.0	50.0	58.0	57.8	547-613 bp	1.0	85.2
trnP-UGG-psaJ_p3	TACCAAACTGCGCTACATCC	AGTACTTACTACCGGTGCCA	50.0	50.0	58.0	57.8	477-543 bp	1.0	85.2
trnP-UGG-psaJ_p4	TCCCTCGTTACTGCTCAGAA	GGAATATCAACGCATCCGGA	50.0	50.0	58.1	57.8	632-698 bp	1.0	84.4
trnP-UGG-psaJ_p5	TCCCTCGTTACTGCTCAGAA	AACCTGCTAAAGACCCGAAC	50.0	50.0	58.1	57.8	587-653 bp	1.0	84.4