Hotspot

psbE-petL

Genome · 68024-69244 · 1221 bp

Back to Crataegus

Pi 0.00424

MarkerSeek score 59.50

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: Genome
length_bp: 1221
variable_sites: 38
pi: 0.00424
unique_haplotype_count: 23
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	AGAACGTTCTCCTGTGCTTC	GTCGTATCTTGCTCAGACCG	50.0	55.0	57.8	58.2	1334-1370 bp	1.0	81.5
psbE-petL_p2	ACGAATAACCAACCCGCAAT	GTCGTATCTTGCTCAGACCG	45.0	55.0	58.2	58.2	1420-1456 bp	1.0	81.1
psbE-petL_p3	GTTCTCCTGTGCTTCCAGAC	GTCGTATCTTGCTCAGACCG	55.0	55.0	58.2	58.2	1329-1365 bp	1.0	81.1
psbE-petL_p4	TGCTGACGAATAACCAACCC	GTCGTATCTTGCTCAGACCG	50.0	55.0	58.2	58.2	1425-1461 bp	1.0	81.0
psbE-petL_p5	AGAACGTTCTCCTGTGCTTC	TCGTATCTTGCTCAGACCGA	50.0	50.0	57.8	58.3	1333-1369 bp	1.0	80.8