Hotspot

petA-psbJ

Genome · 66238-67254 · 1017 bp

Back to Crataegus

Pi 0.00696

MarkerSeek score 44.90

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: Genome
length_bp: 1017
variable_sites: 30
pi: 0.00696
unique_haplotype_count: 16
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 44.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	TGCTCTTCTTGGCATCTGTT	TCCGGATTAGGTTCATCCCT	45.0	50.0	57.4	57.5	1101-1157 bp	1.0	77.7
petA-psbJ_p2	TTGCTCTTCTTGGCATCTGT	TCCGGATTAGGTTCATCCCT	45.0	50.0	57.4	57.5	1102-1158 bp	1.0	77.7
petA-psbJ_p3	TGCTCTTCTTGGCATCTGTT	CCGGATTAGGTTCATCCCTG	45.0	55.0	57.4	57.4	1100-1156 bp	1.0	77.2
petA-psbJ_p4	TTGCTCTTCTTGGCATCTGT	CCGGATTAGGTTCATCCCTG	45.0	55.0	57.4	57.4	1101-1157 bp	1.0	77.2
petA-psbJ_p5	CGTGTCCAAGGTCTTTTGCT	TCCGGATTAGGTTCATCCCT	50.0	50.0	58.7	57.5	1117-1173 bp	1.0	76.7