Hotspot

rpoB-trnC-GCA

Genome · 27810-29019 · 1210 bp

Back to Crataegus

Pi 0.00209

MarkerSeek score 50.80

Primer available yes

Label rpoB-trnC-GCA

Feature Metadata

label_name: rpoB-trnC-GCA
feature_type: igs
region: Genome
length_bp: 1210
variable_sites: 14
pi: 0.00209
unique_haplotype_count: 17
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 50.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC-GCA_p1	TAAGCCCTGCTCAATGAACC	CCGGATTTGAACTGGGGAAA	50.0	50.0	57.9	58.1	1359-1398 bp	1.0	80.8
rpoB-trnC-GCA_p2	TAAGCCCTGCTCAATGAACC	AAAAGGATTTGCAGTCCCCC	50.0	50.0	57.9	58.4	1341-1380 bp	1.0	78.6
rpoB-trnC-GCA_p3	TATTGCCTCATTTCCACCCC	CCGGATTTGAACTGGGGAAA	50.0	50.0	57.5	58.1	1293-1332 bp	1.0	78.3
rpoB-trnC-GCA_p4	CTCTGTTAAGCCCTGCTCAA	CCGGATTTGAACTGGGGAAA	50.0	50.0	57.5	58.1	1365-1404 bp	1.0	78.0
rpoB-trnC-GCA_p5	GTTCCTCTGTTAAGCCCTGC	CCGGATTTGAACTGGGGAAA	55.0	50.0	58.5	58.1	1369-1408 bp	1.0	77.4