Hotspot

trnH-GUG-psbA

Genome · 126-392 · 267 bp

Back to Crataegus

Pi 0.0202

MarkerSeek score 56.10

Primer available yes

Label trnH-GUG-psbA

Feature Metadata

label_name: trnH-GUG-psbA
feature_type: igs
region: Genome
length_bp: 267
variable_sites: 20
pi: 0.0202
unique_haplotype_count: 20
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 56.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	ATATTATGGGCGAACGACGG	CCTCTAGACCTAGCTGCTGT	50.0	55.0	57.9	57.9	378-403 bp	1.0	86.9
trnH-GUG-psbA_p2	GATCCACTTGGCTACATCCG	GCGCTAACCTTGGTATGGAA	55.0	50.0	58.1	58.0	367-392 bp	1.0	86.9
trnH-GUG-psbA_p3	ATATTATGGGCGAACGACGG	GCGCTAACCTTGGTATGGAA	50.0	50.0	57.9	58.0	428-453 bp	1.0	86.8
trnH-GUG-psbA_p4	ATATTATGGGCGAACGACGG	TTCCCTCTAGACCTAGCTGC	50.0	55.0	57.9	57.9	381-406 bp	1.0	86.8
trnH-GUG-psbA_p5	CAATCCACTGCCTTGATCCA	GCGCTAACCTTGGTATGGAA	50.0	50.0	57.9	58.0	381-406 bp	1.0	86.7