Hotspot

ycf1

SSC · 116760-121778 · 5019 bp

Back to Crassula

Pi 0.042

MarkerSeek score 68.30

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5019
variable_sites: 699
pi: 0.042
unique_haplotype_count: 11
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 68.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	TTTTGGTACCAATCCCGACC	GAATCGCCGATTCCAACTCT	50.0	50.0	57.8	58.1	711-720 bp	0.833333	76.5
ycf1_p2	TTTTGGTACCAATCCCGACC	ACAACTGAACAGCCTTCTGG	50.0	50.0	57.8	58.0	999-1008 bp	0.833333	76.2
ycf1_p3	TTTTGGTACCAATCCCGACC	GGGGAAGGACAACTGAACAG	50.0	55.0	57.8	58.1	1007-1016 bp	0.833333	75.6
ycf1_p4	TTTTGGTACCAATCCCGACC	AAATGTACTTGCGACGGTGT	50.0	45.0	57.8	58.1	1203-1212 bp	0.833333	75.1
ycf1_p5	TTTTGGTACCAATCCCGACC	AATGTACTTGCGACGGTGTT	50.0	45.0	57.8	58.1	1202-1211 bp	0.833333	75.1