Hotspot

rps15-ycf1

SSC · 116429-116759 · 331 bp

Back to Crassula

Pi 0.0683

MarkerSeek score 73.20

Primer available yes

Label rps15-ycf1

Feature Metadata

label_name: rps15-ycf1
feature_type: igs
region: SSC
length_bp: 331
variable_sites: 74
pi: 0.0683
unique_haplotype_count: 10
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 73.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps15-ycf1_p1	TCCCAACGCGTTACATAAGG	GAAAGCGCTATGGACGAAGA	50.0	50.0	58.0	58.1	1648-1660 bp	0.833333	75.5
rps15-ycf1_p2	TTACGGTTATGGCTTCGGTG	GAAAGCGCTATGGACGAAGA	50.0	50.0	58.0	58.1	1686-1698 bp	0.833333	75.5
rps15-ycf1_p3	TATTGCCTAACTGTTCCGGC	GAAAGCGCTATGGACGAAGA	50.0	50.0	58.0	58.1	1710-1722 bp	0.833333	75.3
rps15-ycf1_p4	ATGATGAGTCGAAGAACGCC	GAAAGCGCTATGGACGAAGA	50.0	50.0	58.1	58.1	1525-1537 bp	0.833333	75.2
rps15-ycf1_p5	TAACTGTTCCGGCGCATTTA	GAAAGCGCTATGGACGAAGA	45.0	50.0	57.9	58.1	1703-1715 bp	0.833333	74.8