Hotspot

ndhF-rpl32

SSC · 106299-106844 · 546 bp

Back to Crassula

Pi 0.0553

MarkerSeek score 74.90

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 546
variable_sites: 97
pi: 0.0553
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 74.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	TGGAAGTGGAAGCAGAGGTA	TTCGAACCGAGATGCTCAAG	50.0	50.0	58.0	58.0	1218-1299 bp	1.0	91.8
ndhF-rpl32_p2	TGGAAGTGGAAGCAGAGGTA	GGGAGAAAGCAAAATCCCCA	50.0	50.0	58.0	58.1	1018-1053 bp	1.0	91.3
ndhF-rpl32_p3	TGGAAGTGGAAGCAGAGGTA	AAATGCTTTTAACGCTGCCC	50.0	45.0	58.0	57.9	691-725 bp	1.0	90.1
ndhF-rpl32_p4	TGGAAGTGGAAGCAGAGGTA	CAAAATCCCCACCTCCGAAA	50.0	50.0	58.0	58.1	1015-1044 bp	0.833333	78.8
ndhF-rpl32_p5	AGTGGTCGTGTGAACCAAAA	TTCGAACCGAGATGCTCAAG	45.0	50.0	57.9	58.0	1676-1702 bp	0.833333	77.0