Hotspot

psaI-pafII

LSC · 55732-56152 · 421 bp

Back to Crassula

Pi 0.0446

MarkerSeek score 73.20

Primer available yes

Label psaI-pafII

Feature Metadata

label_name: psaI-pafII
feature_type: igs
region: LSC
length_bp: 421
variable_sites: 58
pi: 0.0446
unique_haplotype_count: 10
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 73.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaI-pafII_p1	CTCCATGCTTTCGTTCCCTT	GCCATCACTATCCCTTGTGG	50.0	55.0	58.2	58.0	1112-1246 bp	1.0	90.4
psaI-pafII_p2	GTTTTTCCGGCAATGGCAAT	GCCATCACTATCCCTTGTGG	45.0	55.0	58.2	58.0	557-685 bp	1.0	90.2
psaI-pafII_p3	CTCCATGCTTTCGTTCCCTT	ACGCGCAAGAAATAGGCTAA	50.0	45.0	58.2	58.0	1448-1582 bp	0.833333	77.3
psaI-pafII_p4	GTTTTTCCGGCAATGGCAAT	ACGCGCAAGAAATAGGCTAA	45.0	45.0	58.2	58.0	893-1021 bp	0.833333	76.8
psaI-pafII_p5	TTTTTCCGGCAATGGCAATG	ACGCGCAAGAAATAGGCTAA	45.0	45.0	58.2	58.0	892-1020 bp	0.833333	76.8