Hotspot

rbcL-accD

LSC · 53046-53707 · 662 bp

Back to Crassula

Pi 0.0554

MarkerSeek score 78.70

Primer available yes

Label rbcL-accD

Feature Metadata

label_name: rbcL-accD
feature_type: igs
region: LSC
length_bp: 662
variable_sites: 117
pi: 0.0554
unique_haplotype_count: 10
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 78.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-accD_p1	CGTGAAGCTTGCAAATGGAG	TGTATTTGAACTCCTCCGCG	50.0	50.0	58.0	58.0	810 bp	0.833333	75.7
rbcL-accD_p2	CTGTACTACAGTTCGGTGGC	TGTATTTGAACTCCTCCGCG	55.0	50.0	58.0	58.0	953 bp	0.833333	75.6
rbcL-accD_p3	CTGTACTACAGTTCGGTGGC	GTATTTGAACTCCTCCGCGT	55.0	50.0	58.0	58.0	952 bp	0.833333	75.6
rbcL-accD_p4	AATCACAAGCTGAAACCGGT	TGTATTTGAACTCCTCCGCG	45.0	50.0	58.0	58.0	1466 bp	0.75	71.8
rbcL-accD_p5	AATCACAAGCTGAAACCGGT	GTATTTGAACTCCTCCGCGT	45.0	50.0	58.0	58.0	1465 bp	0.75	71.8