Hotspot

trnT-GGU-psbD

LSC · 30228-30955 · 728 bp

Back to Crassula

Pi 0.0433

MarkerSeek score 75.90

Primer available yes

Label trnT-GGU-psbD

Feature Metadata

label_name: trnT-GGU-psbD
feature_type: igs
region: LSC
length_bp: 728
variable_sites: 102
pi: 0.0433
unique_haplotype_count: 10
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 75.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-GGU-psbD_p1	CGTGAAAGGGAGATGTCCTG	GAACCAACCCCCTAAAGCAA	55.0	50.0	58.0	58.0	1227-1256 bp	1.0	90.3
trnT-GGU-psbD_p2	CATAGTACCCTACCCCCAGG	GAACCAACCCCCTAAAGCAA	60.0	50.0	58.0	58.0	1270-1299 bp	1.0	90.1
trnT-GGU-psbD_p3	CGTGAAAGGGAGATGTCCTG	AAACTAGGCGCAAAGAACCA	55.0	45.0	58.0	58.0	1598-1627 bp	1.0	89.4
trnT-GGU-psbD_p4	CGTGAAAGGGAGATGTCCTG	AACTAGGCGCAAAGAACCAA	55.0	45.0	58.0	58.0	1597-1626 bp	1.0	89.4
trnT-GGU-psbD_p5	CGTGAAAGGGAGATGTCCTG	ACCAAAACTAGGCGCAAAGA	55.0	45.0	58.0	58.0	1602-1631 bp	1.0	89.3