Hotspot

rpoB-trnC-GCA

LSC · 26147-26936 · 790 bp

Back to Crassula

Pi 0.0393

MarkerSeek score 74.90

Primer available yes

Label rpoB-trnC-GCA

Feature Metadata

label_name: rpoB-trnC-GCA
feature_type: igs
region: LSC
length_bp: 790
variable_sites: 105
pi: 0.0393
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 74.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC-GCA_p1	AGTTCTTCCGTCAAGCCATG	CCGGATTTGAACTGGGGAAA	50.0	50.0	58.2	58.1	885-984 bp	1.0	89.9
rpoB-trnC-GCA_p2	GTTCTTCCGTCAAGCCATGA	CCGGATTTGAACTGGGGAAA	50.0	50.0	58.2	58.1	884-983 bp	1.0	89.8
rpoB-trnC-GCA_p3	AAAGTTCTTCCGTCAAGCCA	CCGGATTTGAACTGGGGAAA	45.0	50.0	57.7	58.1	887-986 bp	1.0	88.6
rpoB-trnC-GCA_p4	AGTTCTTCCGTCAAGCCATG	AAAAGGATTTGCAGTCCCCC	50.0	50.0	58.2	58.4	867-966 bp	1.0	87.7
rpoB-trnC-GCA_p5	GTTCTTCCGTCAAGCCATGA	AAAAGGATTTGCAGTCCCCC	50.0	50.0	58.2	58.4	866-965 bp	1.0	87.6