Hotspot

trnS-GCU-trnG-UCC

LSC · 8492-9037 · 546 bp

Back to Crassula

Pi 0.0555
MarkerSeek score 79.90
Primer available yes
Label trnS-GCU-trnG-UCC

Feature Metadata

label_name
trnS-GCU-trnG-UCC
feature_type
igs
region
LSC
length_bp
546
variable_sites
102
pi
0.0555
unique_haplotype_count
10
primer_available
yes
alignment_reliability
0.99
markerseek_score
79.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-GCU-trnG-UCC_p1 CAATCCGACGCTTTAGTCCA TACCACTAAACTATACCCGC 50.0 45.0 58.0 54.0 588-606 bp 1.0 59.9
trnS-GCU-trnG-UCC_p2 AATCCGACGCTTTAGTCCAC TACCACTAAACTATACCCGC 50.0 45.0 58.0 54.0 587-605 bp 1.0 59.9
trnS-GCU-trnG-UCC_p3 CAATCCGACGCTTTAGTCCA ACGAATCTCACTTTTACCACT 50.0 38.1 58.0 54.9 602-620 bp 1.0 59.1
trnS-GCU-trnG-UCC_p4 AATCCGACGCTTTAGTCCAC ACGAATCTCACTTTTACCACT 50.0 38.1 58.0 54.9 601-619 bp 1.0 59.1
trnS-GCU-trnG-UCC_p5 CAATCCGACGCTTTAGTCCA TTACCACTAAACTATACCCGC 50.0 42.9 58.0 54.9 589-607 bp 1.0 58.8